The majority of clinical
P. aeruginosa strains translocates ExoU or ExoS, ExoT and ExoY toxins in the cytoplasm of target cells. We have investigated the effects of these three exotoxins in human endothelial cells and described their role in cell signaling disruption
[1-3].
ExoS – ExoT effect
Live-imaging quantification of PAO1 (ExoS
+) cell infection by fluorescence intensity measurement of Hoechst-labelled nuclei.
Wallez Y.
et al (2018) Scientific Reports [3].
ExoU effect
Live-imaging quantification of PA14 (ExoU
+) cell infection by fluorescence intensity measurement of propidium Iodide incorporation.
References[1] Huber
et al (2014)
Cell Mol Life Sci 71(10):1927-1941.,
[2] Bouillot
et al (2015)
Infection and immunity 83(5):1820-1829.,
[3] Walley et al (2018)
Scientific reports 8(1):5. doi: 10.1038/s41598-017-18501-9.